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Scarless skin repair in immunodeficient mice
Author(s) -
GawronskaKozak Barbara,
Bogacki Marek,
Rim JongSeop,
Monroe W. Todd,
Manuel Jessica A.
Publication year - 2006
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1743-6109.2006.00121.x
Subject(s) - masson's trichrome stain , hydroxyproline , pathology , immunohistochemistry , h&e stain , medicine , trichrome , cd8 , nude mouse , wound healing , staining , flow cytometry , regeneration (biology) , histology , immune system , immunology , biology , microbiology and biotechnology , cancer
Scarring, the end result of the wound healing process in adult mammals, is a problem of significant clinical importance. We observed that athymic nude‐ nu mice, similar to mammalian fetuses, are able to restore the structure and integrity of injured skin through a process resembling regeneration, where scar formation is absent. Among the postinjured skin tissues collected from athymic nude‐ nu , wild‐type controls (C57BL/6J), severe‐combined immunodeficient, Rag (lack of B and T cells), athymic (thymectomized neonates and adult C57BL/6J), and mice treated with an immunosuppressant (cyclosporin A), only athymic nude‐ nu mice showed: a lack of scar by histological examination (hematoxylin & eosin and Masson's trichrome staining), low levels of collagen (as determined by hydroxyproline content), high levels of hyaluronic acid, a statistically significant increase in elastic modulus for injured samples over unwounded (biomechanical testing) and low levels of the pro‐scarring cytokines platelet‐derived growth factor‐B and transforming growth factor β1. Additionally, immunohistochemical and Western blot analyses of postinjured tissues as well as flow cytometry analysis of blood samples showed the presence of CD8‐positive cells in all studied animals except nude‐ nu mice. We conclude that scarless skin healing in athymic nude‐ nu mice provides a new model to study the influence of the immune system on tissue regeneration.