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Investigation of Biological Activities of Dichloromethane and Ethyl Acetate Fractions of P latonia insignis Mart. Seed
Author(s) -
Costa Júnior Joaquim S.,
Ferraz Alexandre B.F.,
Sousa Taciana O.,
Silva Romézio A.C.,
Lima Sidney G.,
Feitosa Chistiane M.,
Citó Antônia M.G.L.,
Melo Cavalcante Ana A.C.,
Freitas Rivelilson M.,
Moura Sperotto Angelo R.,
Péres Valéria F.,
Moura Dinara J.,
Saffi Jenifer
Publication year - 2013
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2012.00924.x
Subject(s) - dichloromethane , ethyl acetate , traditional medicine , chemistry , botany , biology , chromatography , organic chemistry , medicine , solvent
P latonia insignis M art., a native species of the B razilian A mazon more commonly known as bacuri, is a member of the C lusiaceae family. In this study, we evaluated the chemical composition and the antioxidant and toxicity activities of the dichloromethane and ethyl acetate fractions from P . insignis seed ethanolic extract using different experimental models. Our results demonstrate in vitro antioxidant effects, by 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid) diammonium salt and 1,1‐diphenyl‐2‐picryl‐hydrazyl assays, as well as in vivo effects in antioxidant‐defective S accharomyces cerevisiae strains to both fractions. Toxicity was evaluated against the micro‐crustaceous A rtemia salina L each. and promastigote L eishmania amazonensis . The dichloromethane fraction was the most active fraction evaluated on A . salina and promastigote L . amazonensis (IC 50 = 24.89 μg/mL and 2.84 μg/mL, respectively). In addition, a slight cytotoxicity was observed in mammalian V 79 cells using ethyl acetate and dichloromethane fractions with MTT assays. Both fractions displayed genotoxicity up to 25 μg/mL (dichloromethane) and 10 μg/mL (ethyl acetate) in V 79 cells, as evaluated by the alkaline comet assay. Thus, in this study, we demonstrate for the first time that ethyl acetate and dichloromethane fractions from P . insignis seeds display antioxidant effects, a toxic effect against A . salina and L . amazonensis and induce genotoxicity in V 79 mammalian cells. The observed activities can be attributed to the phenolic compounds present in these fractions and to the presence of xanthones (alpha‐ and gamma‐mangostin).