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Dynamic Oxidative Stress and DNA Damage Induced by Oestrogen Deficiency and Protective Effects of P uerarin and 17β‐ O estradiol in Ovariectomized Rats
Author(s) -
Tang XuLei,
Liu XiaoJu,
Tian Quan,
Zhang Wei
Publication year - 2012
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2012.00864.x
Subject(s) - ovariectomized rat , puerarin , oxidative stress , endocrinology , dna damage , malondialdehyde , medicine , comet assay , estrogen , phytoestrogens , chemistry , pharmacology , dna , biochemistry , alternative medicine , pathology
Oxidative stress plays an essential role in the pathogenesis of cardiovascular diseases and osteoporosis resulting from oestrogen deficiency in the postmenopausal period. In this report, we observed a dynamic change of oxidative stress and DNA damage after ovariectomy in female rats. We then compared phytoestrogen puerarin and 17β‐oestradiol (E 2 ) in their effects on oestrogen deficiency‐induced oxidative stress and DNA damage. Serum total antioxidant capacity ( TAC ), malondialdehyde ( MDA ) and lymphocytes DNA damage (comet%) were measured. There was a gradual increase in oxidative stress in the ovariectomized ( OVX ) rats over time after ovariectomy, as compared to rats receiving sham operation. OVX rats that were on puerarin and E 2 showed increased TAC and decreased MDA in the serum, as well as decreased lymphocytes comet%. P uerarin appeared to have a more powerful protective effect on DNA oxidative damage than E 2 . The study indicates that postmenopausal women may benefit from phytoestrogen puerarin.