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Bioassay‐guided Evaluation of Antioxidant and Antinociceptive Activities of Carvacrol
Author(s) -
Guimarães Adriana G.,
Oliveira Geovana F.,
Melo Mônica S.,
Cavalcanti Sócrates C.H.,
Antoniolli Angelo R.,
Bonjardim Leonardo R.,
Silva Francilene A.,
Santos João Paulo A.,
Rocha Ricardo F.,
Moreira José Claudio F.,
Araújo Adriano A.S.,
Gelain Daniel P.,
QuintansJúnior Lucindo J.
Publication year - 2010
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2010.00609.x
Subject(s) - chemistry , licking , nociception , antioxidant , pharmacology , lipid peroxidation , opioid , nitric oxide , anesthesia , capsaicin , hot plate test , medicine , biochemistry , receptor , organic chemistry
We examined the antioxidant properties in vitro and the antinociceptive effect of carvacrol (CARV) in several models of pain in mice. CARV presented a strong antioxidant potential according to the TRAP/TAR evaluation; it also presented scavenger activity against nitric oxide and prevented lipid peroxidation in vitro . In mice, when evaluated against acetic acid‐induced abdominal writhing, CARV (25, 50 and 100 mg/kg, i.p.) reduced ( p < 0.001) the number of writhing compared to the control group, without opioid participation. In the formalin test, CARV also significantly inhibited both the early (neurogenic pain) and the late (inflammatory pain) phases of formalin‐induced licking, with inhibition percentage values of 56.8% (100 mg/kg) for the neurogenic phase and 41.2% (25 mg/kg), 73.8% (50 mg/kg) and 99.7% (100 mg/kg) for the inflammatory phase. CARV also produced a significant inhibition of the pain caused by capsaicin (63.1, 67.1 and 95.8%, p < 0.001) and glutamate (46.4, 61.4 and 97.9%, p < 0.01). When assessed in a thermal model of pain, CARV (100 mg/kg, i.p.) caused a significant increase ( p < 0.05) in the latency response on the hot‐plate test. Such results were unlikely to be provoked by motor abnormality. Together, these results indicate that the properties of CARV should be more thoroughly examined in order to achieve newer tools for management and/or treatment of painful conditions, including those related to pro‐oxidant states.