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Dynamic Epigenetic Changes Involved in Testicular Toxicity Induced by Di‐2‐(Ethylhexyl) Phthalate in Mice
Author(s) -
Wu Shengde,
Zhu Jing,
Li Yasha,
Lin Tao,
Gan Liqiang,
Yuan Xingang,
Xiong Jing,
Liu Xing,
Xu Mingdeng,
Zhao Dan,
Ma Chao,
Li Xuliang,
Wei Guanghui
Publication year - 2010
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2009.00483.x
Subject(s) - phthalate , epigenetics , dna methylation , methylation , methyltransferase , toxicity , biology , blot , andrology , dna , endocrinology , medicine , chemistry , genetics , gene expression , gene , organic chemistry
The aim of this study was to analyse epigenetic (specifically, DNA methylation) change in testes induced by maternal exposure to di‐2‐(ethylhexyl) phthalate (DEHP) in mice. Testicular dysgenesis syndrome was induced in foetuses by maternal exposure to DEHP. High‐performance liquid chromatography was performed to analyse DNA methylation status, and expression levels of the DNA methyltransferases were examined by quantitative real‐time polymerase chain reaction and western blotting. DEHP significantly had more than 10% relative increase in the global DNA methylation and also increased DNA methyltransferases’ expression. Changes in DNA methylation may play an important role in abnormal testicular function caused by environmental factors such as maternal exposure to DEHP, which may be one possible mechanism of DEHP‐mediated testicular toxicity.