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New Haemolytic Potential Assay on an Alternative Insect Model
Author(s) -
Berger Josef
Publication year - 2009
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2009.00446.x
Subject(s) - haemolysis , phenylhydrazine , hemolymph , in vivo , biology , xenobiotic , spodoptera , toxicology , chemistry , immunology , biochemistry , microbiology and biotechnology , enzyme , medicinal chemistry , gene , recombinant dna
A new screening test of a haemolytic potential is presented on Spodoptera larvae in vivo . The assay was evaluated using phenylhydrazine, the well‐known xenobiotic which induces haemolysis in mammals. Haemocyte disintegration causes changes in total haemocyte counts and differentials. It is shown that only qualitative evaluation of panoptically stained haemolymph smears is sufficient, fast and cheap to detect haemolytic potential. The presented assay also prevents unneeded, non‐ethical use of vertebrates.