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Nitric Oxide‐Releasing Compounds Inhibit the Production of Interleukin‐2, ‐4 and ‐10 in Activated Human Lymphocytes
Author(s) -
Korhonen Riku,
Kosonen Outi,
Hämäläinen Mari,
Moilanen Eeva
Publication year - 2008
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2008.00275.x
Subject(s) - nitric oxide , concanavalin a , chemistry , phorbol , endocrinology , biochemistry , medicine , microbiology and biotechnology , biology , enzyme , protein kinase c , in vitro
In the present study, we investigated the effects of nitric oxide donors, GEA 3162 (1,2,3,4‐oxatriazolium,5‐amino‐3(3,4‐dichlorophenyl)‐chloride), GEA 3175 (1,2,3,4‐oxatriazolium,3‐(3‐chloro‐2‐methylphenyl)‐5‐[[(4‐methylphenyl) sulfonyl]amino]‐, hydroxide inner salt) and S‐nitroso‐N‐acetylpenicillamine (SNAP), on the production of Th1 [interleukin (IL)‐2] and Th2 (IL‐4 and IL‐10) type cytokines in activated human lymphocytes. Lymphocytes were stimulated with concanavalin A or a combination of thapsigargin and phorbol myristate acetate in the absence or in the presence of nitric oxide donors. Concanavalin A induced expression of IL‐2 mRNA and production of IL‐2, and the combination of thapsigargin and phorbol myristate acetate induced expression of IL‐4 and IL‐10 mRNAs and production of IL‐4 and IL‐10. These effects were inhibited by the nitric oxide donors in a dose‐dependent manner, GEA 3162 and GEA 3175 being more potent than SNAP on a molar basis. The results show that nitric oxide donors have immunomodulatory properties in both Th1‐ and Th2‐derived responses.