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Effects on Lipid Peroxidation and Antioxidative Enzymes of Euonymus alatus in Cultured Rat Hepatocytes
Author(s) -
Kim KyungWoon,
Suh SeokJong,
Kim JongDae,
Kim SooSung,
Lee InSeon,
Kim JuneKi,
Chang GyuTae,
Kim DongSoo,
Kim CheorlHo
Publication year - 2009
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2007.00152.x
Subject(s) - lipid peroxidation , superoxide dismutase , chemistry , antioxidant , biochemistry , euonymus , glutathione peroxidase , catalase , glutathione , hepatocyte , oxidative stress , malondialdehyde , pharmacology , enzyme , biology , in vitro , paleontology
The Euonymus alatus (Thunb.) Sieb. has long been used as a crude drug. In this paper, we investigate the effects of E. alatus on cultured hepatocyte cell system and lipid peroxidation in hydrogen peroxide (H 2 O 2 ) treatment conditions. The study covers the physiological activity (the antioxidative activity and the nitrite‐scavenging effect) of E. alatus . H 2 O 2 that can produce intracellular free radical was used for inducer of the peroxidation of cellular lipids. Treatment of E. alatus attenuated in cell killing enhanced by increasing concentrations of H 2 O 2 . The increased malondialdehyde level induced by H 2 O 2 treatment was reduced by pre‐treatment of E. alatus . Furthermore, addition of E. alatus in cell culture medium significantly reduced cell killing and content of intracellular antioxidants. Changes in nitrite‐scavenging effect of E. alatus at various concentrations (5–25 mg/ml) and various pH levels (pH 1.2, 4.2 and 6.0) were also observed. The present study was also done to investigate the effects of E. alatus on cultured hepatocyte cell system, H 2 O 2 ‐induced cytotoxicity and antioxidative enzyme activities, including catalase, superoxide dismutase, glutathione peroxidase and glutathione S‐transferase in H 2 O 2 treatment conditions. E. alatus treatment had significant protective or elevating activities on these antioxidative enzyme activities compared to a normal group. The results indicate that E. alatus provides a strong antioxidant protection of cells against H 2 O 2 ‐induced oxidative stress.