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Torsion/Detorsion of the Testis Does Not Modify Responses to Nitric Oxide in Rat Isolated Penile Bulb
Author(s) -
Ozkan Melike H.,
Vural İ. Mert,
Moralioglu Serdar,
Uma Serdar,
Sarioglu Yusuf
Publication year - 2007
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2007.00087.x
Subject(s) - sodium nitroprusside , papaverine , nitric oxide , medicine , penis , stimulation , anesthesia , sildenafil , phenylephrine , chemistry , endocrinology , anatomy , blood pressure
Ischaemia‐reperfusion damage induced by torsion/detorsion of the testicles may be a causative factor leading to erectile dysfunction through oxidative stress‐dependent changes in the responses of the penile bulb, an erectile tissue of the penis. We aimed at investigating the effects of unilateral testicular torsion/detorsion (2 or 24 hr) treatment on relaxations induced by electrical field stimulation and sodium nitroprusside in rat isolated penile bulb. Male Sprague‐Dawley rats used in the study were divided into two groups. The treatment group was subjected to unilateral torsion followed by detorsion for 2 or 24 hr, while the control group underwent only sham operation. For in vitro organ bath experiments, penile bulbs were isolated and responses to relaxant agents and electrical field stimulation (70 V, 1 msec., 0.5–8 Hz, 5 sec.) were recorded on a computer‐based data acquisition system via a force displacement transducer. In tissues precontracted with phenylephrine (3 × 10 −6 M), relaxations induced by electrical field stimulation were not significantly different before and after 2 or 24 hr of detorsion. Similarly sodium nitroprusside‐ (10 −8 –3 × 10 −6 M) and papaverine‐induced (10 −7 –10 −4 M) relaxations were also found unchanged in the detorsion group compared to control. In conclusion, spermatic cord torsion did not lead to impairment in nitric oxide‐mediated relaxant responses of the rat isolated penile bulb.