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Protective Effects of Caffeic Acid Phenethyl Ester, Vitamin C, Vitamin E and N ‐Acetylcysteine on Vancomycin‐Induced Nephrotoxicity in Rats
Author(s) -
Ocak Sabahattin,
Gorur Sadik,
Hakverdi Sibel,
Celik Sefa,
Erdogan Suat
Publication year - 2007
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2007.00051.x
Subject(s) - vancomycin , caffeic acid phenethyl ester , nephrotoxicity , acetylcysteine , vitamin , vitamin e , pharmacology , chemistry , medicine , toxicity , antioxidant , biochemistry , caffeic acid , biology , staphylococcus aureus , bacteria , genetics
The objective of this study was to compare the beneficial effects of caffeic acid phenethyl ester (CAPE), vitamin C, vitamin E and N ‐acetylcysteine on vancomycin‐induced nephrotoxicity. Thirty rats were randomly devided into six groups: (i) control; (ii) vancomycin, 200 mg/kg administrated via intraperitoneal route; (iii) vancomycin plus CAPE – vancomycin with 10 µmol/kg CAPE; (iv) vancomycin plus vitamin C – vancomycin (intraperitoneally) with 200 mg/dl vitamin C in drinking water; (v) vancomycin plus vitamin E – vancomycin with 1000 mg/kg body weight vitamin E (intramuscularly); and (vi) vancomycin plus N ‐acetylcysteine – vancomycin with 10 mg/kg body weight (intraperitoneally) of N ‐acetylcysteine. Vancomycin treatments were started 1 day after the first administrations of these agents and continued for 7 days. At the end of the experiments, catalase activity was significantly decreased by vancomycin in kidney homogenates (P < 0.05). Vitamin E, vitamin C, N ‐acetylcysteine and CAPE administrations decreased the blood urea nitrogen levels increased by vancomycin, although significant differences were detected only in the vitamins E and C groups (P < 0.05). Increased renal malondialdehyde and nitric oxide levels by vancomycin were significantly suppressed by agents used in the study (P < 0.05). Histopathological examination demonstrated prominent damages in the vancomycin‐treated group. Vitamin E was the most beneficial agent on vancomycin‐induced tubular damage, followed by vitamin C, N ‐acetylcysteine and CAPE treatments, respectively. The data suggest that vitamin E, as well as vitamin C, N ‐acetylcysteine and CAPE, could be useful for reducing the detrimental effects on vancomycin‐induced toxicity in kidneys.