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Effects of Daidzein Sulfates on Blood Pressure and Artery of Rats
Author(s) -
Cao YongXiao,
Yang XiaoJiang,
Liu Jing,
Li KeXi
Publication year - 2006
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2006.pto_565.x
Subject(s) - daidzein , contraction (grammar) , chemistry , myograph , medicine , isometric exercise , vasodilation , blood pressure , endocrinology , extracellular , biochemistry , genistein
The aim of this study is to investigate the hypotensive and vasodilator effects of daidzein sulfates, a water‐solubility derivative of daidzein. Tail cuff blood pressure of spontaneously hypertensive rat (SHR) was measured with non‐invasive Electro‐Sphygmomanometer. An isometric tension of rat mesenteric artery ring segments was recoded in vitro on a myograph. The results showed that daidzein sulfates (20 and 40 mg/kg) could decrease blood pressure of SHR in single dose and multi‐doses. Daidzein sulfates (1–100 μM) inhibited the contraction of rat mesenteric arterial ring segments induced by norepinephrine (NA) and 5‐hydroxytryptamine (5‐HT). Daidzein sulfates (100–1000 μM) inhibited arterial segment's contraction induced by KCl and CaCl 2 . The concentration‐ contractive curves were shifted toward right in a non‐parallel manner with decreased E max. Daidzein sulfaltes inhibited the extracellular Ca 2+ ‐dependent contraction. Daidzein sulfates of 100 and 300 μM significantly inhibited the contraction induced by CaCl 2 in Ca 2+ ‐free solution, which is an extracellular Ca 2+ ‐dependent contraction; but daidzein sulfates did not inhibit the intracellular Ca 2+ ‐dependent NA‐induced contraction, in Ca 2+ ‐free solution. The results suggest that daidzein sulfates possess significant hypotensive and vasodilator effects which mainly derive from artery smooth muscle cells by inhibiting the receptor‐mediated Ca 2+ ‐influx.