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Isolation, Partial Purification and Characterization of Active Polypeptide from the Sea Anemone Bartholomea annulata
Author(s) -
SánchezRodríguez Judith,
Zugasti Alejandro,
Santamaría Abel,
GalvánArzate Sonia,
SeguraPuertas Lourdes
Publication year - 2006
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2006.pto_428.x
Subject(s) - sea anemone , cnidocyte , distilled water , anemone , median lethal dose , chemistry , chromatography , biology , dandelion , toxicity , botany , cnidaria , medicine , ecology , alternative medicine , organic chemistry , coral , traditional chinese medicine , pathology
In the sea anemone Bartholomea annulata , four different types of cnidocysts, basitrichous isorhizas, microbasic p‐mastigophores, microbasic amastigophores and spirocysts were identified. In relation to the efficacy of different substances to induce discharge of nematocysts we observe that distilled water induced more than 70% of microbasic p‐mastigophores to discharge, whereas spirocysts were discharged in a lesser extent (≈20%). The median lethal dose (LD 50 ) in mice was found after injection of 700.7 mg protein per kg of body weight from the crude extract. The protein with neurotoxic effect was isolated using low‐pressure liquid chromatography. The neurotoxic activity was determined using sea crabs ( Ocypode quadrata ), injecting 15 μg of crude extract or isolated fraction into the third walking leg, and violent motor activity followed by progressive loss of sensibility to external stimuli, further leading to full paralysis were observed. The active fraction (called V) eluted at 43.9 min.