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Aflatoxin B 1 ‐Induced DNA Damage in Labeo rohita : Protective Effect of an Antioxidant Supplement, Amrita Bindu
Author(s) -
Madhusudhanan N.,
Kavithalakshmi S. N.,
Shanmugasundaram E. R. B.,
Shanmugasundaram K. R.
Publication year - 2006
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2006.pto_346.x
Subject(s) - aflatoxin , antioxidant , chemistry , labeo , dna , adduct , dna adduct , dna damage , food science , dna fragmentation , mycotoxin , agarose gel electrophoresis , biochemistry , biology , fish <actinopterygii> , programmed cell death , organic chemistry , apoptosis , fishery
The present study was undertaken to investigate the effect of potent hepatocarcinogen aflatoxin B 1 in adduct formation and DNA damage in Labeo rohita. Also, the salubrious efficacy of an antioxidant supplement Amrita Bindu (based on Indian system of Medicine) was investigated. Fish weighing 175–250 g were administered intraperitoneally a single dose of 100 μg aflatoxin B 1 /100 g body wt. and another group was given 20% solution of Amrita Bindu along with aflatoxin B 1 at 100 μg/100 g body wt. On the 3rd and 6th day, the liver tissue was analyzed for aflatoxin concentration, aflatoxin‐DNA adduct formation and DNA damage measured in terms of single strand breaks. The fishes administered with aflatoxin B 1 showed elevated concentration of aflatoxin along with a parallel increase in the DNA adduct when compared with the controls. While the fish co‐administered with Amrita Bindu showed 34% and 24% reduction in aflatoxin deposition (accumulation) and aflatoxin‐DNA adduct formation respectively on the 3rd day, a further reduction by around 41% and 33% in aflatoxin deposition and DNA adduct formation respectively was observed on the 6th day. Furthermore, the increased single strand breaks (measured by alkaline single cell gel assay) and fragmentation observed in agarose gel electrophoresis in aflatoxin B 1 administered fish were significantly reduced by Amrita Bindu co‐administration. In conclusion, this is the first report to show aflatoxin B 1 ‐induced DNA adduct formation and DNA damage in one of the major Indian culturable fish, Labeo rohita. Also, our observations show that the antioxidant supplement, Amrita Bindu , has a potential role in ameliorating the aflatoxin B 1 ‐induced DNA damage thus suggesting its applicability in preventing the vital macromolecule DNA.