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Dual Effect of Flurbiprofen on Cell Proliferation and Agonist‐Induced Ca 2+ Movement in Human Osteosarcoma Cells
Author(s) -
Tseng LiLing,
Cheng HeHsiung,
Huang ChunJen,
Liu ShiuhInn,
Kuo ChunChi,
Chen WeiChuan,
Huang JongKhing,
Hsu ShuShong,
Chang HongTai,
Kao ChengHsing,
Ho ChinMan,
Jan ChungRen
Publication year - 2006
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2006.pto_279.x
Subject(s) - flurbiprofen , thapsigargin , chemistry , endoplasmic reticulum , histamine , extracellular , intracellular , endocrinology , medicine , cytosol , pharmacology , biology , biochemistry , enzyme
In human MG63 osteosarcoma cells, the effect of flurbiprofen on intracellular Ca 2+ concentrations ([Ca 2+ ] i ) and proliferation was explored. The proliferation was enhanced by 20–120 μM flurbiprofen, and was decreased by 140–200 μM flurbiprofen. The effect of flurbiprofen on the increases in cytosolic free Ca 2+ levels ([Ca 2+ ] i ) induced by ATP, bradykinin, histamine and thapsigargin (an inhibitor of the endoplasmic reticulum Ca 2+ ATPase), was examined. In cell preincubated with 20 or 80 μM flurbiprofen, the [Ca 2+ ] i increases induced by all agonists were attenuated. In the presence of 20 μM flurbiprofen, the decreased [Ca 2+ ] i responses with the agonists were attributed to a defective Ca 2+ influx because this decrease was unobserved in agonists‐induced [Ca 2+ ] i increases in the absence of extracellular Ca 2+ . In the presence of 80 μM flurbiprofen, both the Ca 2+ influx component and the Ca 2+ releasing (from organelles) component were defective. These results suggest that flurbiprofen could alter proliferation and inhibit [Ca 2+ ] i increases.