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Effects of Amifostine on Glycerol‐Pretreated Rabbit Kidneys
Author(s) -
Barun Süreyya,
Ertoy Dilek,
Dileköz Ergin,
Müftüogˇlu Sevda,
Erten Yasemin,
Sucak Gülsan,
Bali Musa,
Tekeli Nurçin,
Sarıogˇlu Yusuf,
Ercan Zeynep Sevim
Publication year - 2005
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2005.pto_97389.x
Subject(s) - amifostine , glycerol , chemistry , vasodilation , pharmacology , perfusion , vasoconstriction , endocrinology , medicine , biochemistry , toxicity , organic chemistry
Glycerol‐induced acute renal failure is an experimental model for myoglobinuric nephropathy. Amifostine is a cytoprotective agent which scavenges the free radicals. Since there is enhanced production of reactive oxygen metabolites in glycerol‐induced acute renal failure, we wanted to examine whether amifostine has a protective role against vascular reactivity and histological changes in kidneys isolated from glycerol‐pretreated rabbits. Perfusion pressure was recorded from kidneys obtained from rabbits injected with glycerol 3 hr before the experiments and from glycerol‐pretreated and non‐pretreated rabbits injected with amifostine 30 min. before the experiments. Acetylcholine‐induced (10 −8 ‐10 −5 M) vasodilatation was tested following the construction of submaximal vasoconstriction by phenylephrine. Histological investigation was performed using light microscope. Acetylcholine‐induced vasodilatation was found to be significantly decreased in glycerol, glycerol+amifostine and amifostine groups compared to controls at all concentrations. Reduction in acetylcholine‐induced vasodilation was more prominent in amifostine group compared to amifostine+glycerol group. There was histological renal damage in all experimental groups and this damage was more pronounced in glycerol+amifostine group. In conclusion, contrary to expectation, amifostine per se led to histological damage and potentiated the histological damage caused by glycerol and produced a decrease in acetylcholine‐induced vasodilatation. The mechanisms by which amifostine exerts its effects are not known.