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Inhibition of the Protease Activity of the Light Chain of Type A Botulinum Neurotoxin by Aqueous Extract from Stinging Nettle ( Urtica dioica ) Leaf
Author(s) -
Gul Nizamettin,
Ahmed S. Ashraf,
Smith Leonard A.
Publication year - 2004
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2004.pto950503.x
Subject(s) - protease , botulinum neurotoxin , urtica dioica , chemistry , neurotoxin , urticaceae , immunoglobulin light chain , biochemistry , enzyme , protease inhibitor (pharmacology) , chromatography , botany , biology , toxin , antibody , virology , immunology , human immunodeficiency virus (hiv) , viral load , antiretroviral therapy
We investigated the inhibitory effect of stinging nettle leaf extract on the protease activity of botulinum neurotoxin type A and B light chains. The nettle leaf infusion was fractionated and HPLC‐based enzymatic assays were performed to determine the capacity of each fraction to inhibit the protease activity of botulinum neurotoxin type A and B light chains. Assay results demonstrated that a water‐soluble fraction obtained from the nettle leaf infusion inhibited type A, but did not inhibit type B light chain protease activity. The inhibition mode of water soluble fraction against protease activity of type A light chain was analyzed and found to be a non‐competitive.