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The Influence of Amphotericin B and Neomycin on the Effect of Human Relaxin‐2 on Foetal Membranes and Isolated Myometrium
Author(s) -
Vogel Ida,
Grønbæk Henning,
Uldbjerg Niels,
Forman Axel
Publication year - 2004
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2004.pto940308.x
Subject(s) - myometrium , neomycin , contractility , medicine , endocrinology , biology , amphotericin b , pharmacology , chemistry , microbiology and biotechnology , uterus , antibiotics , antifungal
In vitro studies have documented effects of relaxin on utero‐placental tissues. Previously unpublished experiments indicate that neomycin and amphotericin B in vitro influences the effect of human relaxin‐2 on the strength of human foetal membranes. The aim of the current study was to investigate the interaction between neomycin and amphotericin B and human relaxin‐2 using human foetal membranes, human myometrium and rat myometrium. Chloramphenicol, erythromycin and penicillin were also examined. Human foetal membranes were stretched until rupture in a materials‐testing machine while the contractility of rat and human myometrium were examined by myography. Human relaxin‐2 (hrlx‐2, 10 −9 M) induced a decreased strength in human foetal membranes, although this effect of hrlx‐2 was inhibited after co‐incubation with neomycin and amphotericin B. Hrlx‐2 (10 −9 M) in combination with chloramphenicol induced a decreased strength of human foetal membranes. Hrlx‐2 (10 −9 M) decreased myometrial contractility on amplitude and frequency in the rat myometrium, but had no effect on baseline tension. After exposure to amphotericin B, hrlx‐2 induced a more pronounced decrease in amplitude, increased baseline tension and increased the frequency of contractions of the rat myometrium. Hrlx‐2 (10 −9 M) had no effect on the human myometrium. However, after exposure to amphotericin B or neomycin+amphotericin B, hrlx‐2 induced an increase in baseline tension and a decrease in amplitude. Amphotericin B and neomycin+amphotericin B increased the frequency of contractions and this effect was further enhanced by the addition of hrlx‐2. We therefore conclude that amphotericin B and neomycin + amphotericin B may have an influence on relaxin's effect on isolated foetal membranes and myometrium.