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Microglial Calcium Responses to Platelet‐Activating Factor are Inhibited by Analogue CAS 99103‐16‐9 and Dihydropyridine PCA 4248 but Not by Ginkgolide A
Author(s) -
Ranasinghe Saman,
Bolsover Stephen
Publication year - 2004
Publication title -
basic and clinical pharmacology and toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 90
eISSN - 1742-7843
pISSN - 1742-7835
DOI - 10.1111/j.1742-7843.2004.950208.x
Subject(s) - calcium , dihydropyridine , chemistry , platelet , platelet activating factor , medicine , endocrinology , pharmacology , organic chemistry
Calcium signals evoked in N9 microglial cells were monitored using the calcium indicator dye Fluo‐4 in a fluorescence imaging plate reader. Platelet activating factor in the range 100 nM to 20 μM elicited graded calcium responses. The analogue CAS 99103‐16‐9 inhibited the evoked calcium rise with an apparent K B of 1.3±0.4 μM. The dihydropyridine PCA 4248 inhibited the evoked calcium rise with an apparent K B of 1.2±0.2 μM. Ginkgolide A at concentrations up to 18 μM had no effect on the evoked calcium rise. While CAS 99103‐16‐9 and PCA 4248 appear to be simple competitive inhibitors of platelet‐activating factor responses, the efficacy of ginkgolide in more complex pharmacological situations may result from an action at a site other than the platelet‐activating factor receptor.