Open AccessTGF ‐β 1 expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding
Author(s) -
Alves Claudia Cristina,
Torrinhas Raquel Susana,
Giorgi Ricardo,
Brentani Maria Mitzi,
Logullo Angela Flavia,
Waitzberg Dan Linetzky
Publication year - 2014
Publication title -
international wound journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.867
H-Index - 63
eISSN - 1742-481X
pISSN - 1742-4801
DOI - 10.1111/j.1742-481x.2012.01120.x
Subject(s) - enteral administration , medicine , wound healing , malnutrition , granulation tissue , parenteral nutrition , saline , messenger rna , endocrinology , growth factor , gastroenterology , andrology , surgery , biochemistry , gene , biology , receptor
Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post‐trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT‐PCR). M(C) rats had significantly lower transforming growth factor β ( TGF‐β 1 ) mRNA levels than E(C) rats (2·58 ± 0·83 versus 3·53 ± 0·57, P < 0·01) and in comparison with M(S) and M(A) rats (4·66 ± 2·49 and 4·61 ± 2·11, respectively; P < 0·05). VEGF and KGF‐7 mRNA levels were lower in M(A) rats than in E(A) rats (0·74 ± 0·16 versus 1·25 ± 0·66; and 1·07 ± 0·45 versus 1·79 ± 0·89, respectively; P ≤ 0·04), but did not differ from levels in E(C) and M(C) animals. In experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF‐β 1 genes, which was minimised by early enteral feeding with standard or supplemented diets.