Premium
Germinal center‐specific protein human germinal center associated lymphoma directly interacts with both myosin and actin and increases the binding of myosin to actin
Author(s) -
Lu Xiaoqing,
Kazmierczak Katarzyna,
Jiang Xiaoyu,
Jones Michelle,
Watt James,
Helfman David M.,
Moore Jeffrey R.,
SzczesnaCordary Danuta,
Lossos Izidore S.
Publication year - 2011
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2011.08109.x
Subject(s) - myosin , germinal center , actin , motility , microbiology and biotechnology , myosin head , biology , myosin light chain kinase , chemistry , antibody , b cell , genetics
Human germinal center associated lymphoma (HGAL) is a germinal center‐specific gene whose expression correlates with a favorable prognosis in patients with diffuse large B‐cell and classic Hodgkin lymphomas. HGAL is involved in negative regulation of lymphocyte motility. The movement of lymphocytes is directly driven by actin polymerization and actin–myosin interactions. We demonstrate that HGAL interacts directly and independently with both actin and myosin and delineate the HGAL and myosin domains responsible for the interaction. Furthermore, we show that HGAL increases the binding of myosin to F‐actin and inhibits the ability of myosin to translocate actin by reducing the maximal velocity of myosin head/actin movement. No effects of HGAL on actomyosin ATPase activity and the rate of actin polymerization from G‐actin to F‐actin were observed. These findings reveal a new mechanism underlying the inhibitory effects of germinal center‐specific HGAL protein on lymphocyte and lymphoma cell motility.