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Oligomannose‐coated liposomes efficiently induce human T‐cell leukemia virus‐1‐specific cytotoxic T lymphocytes without adjuvant
Author(s) -
Kozako Tomohiro,
Hirata Shinya,
Shimizu Yoshitaka,
Satoh Yuichiro,
Yoshimitsu Makoto,
White Yohann,
Lemonnier François,
Shimeno Hiroshi,
Soeda Shinji,
Arima Naomichi
Publication year - 2011
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2011.08055.x
Subject(s) - cytotoxic t cell , epitope , biology , major histocompatibility complex , antigen , cd8 , t cell , immunology , virology , antigen presenting cell , immune system , biochemistry , in vitro
Human T‐cell leukemia virus‐1 (HTLV‐1) causes adult T‐cell leukemia/lymphoma, which is an aggressive peripheral T‐cell neoplasm. Insufficient T‐cell response to HTLV‐1 is a potential risk factor in adult T‐cell leukemia/lymphoma. Efficient induction of antigen‐specific cytotoxic T lymphocytes is important for immunological suppression of virus‐infected cell proliferation and oncogenesis, but efficient induction of antigen‐specific cytotoxic T lymphocytes has evaded strategies utilizing poorly immunogenic free synthetic peptides. Here, we examined the efficient induction of an HTLV‐1‐specific CD8+ T‐cell response by oligomannose‐coated liposomes (OMLs) encapsulating the human leukocyte antigen (HLA)‐A*0201‐restricted HTLV‐1 Tax‐epitope (OML/Tax). Immunization of HLA‐A*0201 transgenic mice with OML/Tax induced an HTLV‐1‐specific gamma‐interferon reaction, whereas immunization with epitope peptide alone induced no reaction. Upon exposure of dendritic cells to OML/Tax, the levels of CD86, major histocompatibility complex class I, HLA‐A02 and major histocompatibility complex class II expression were increased. In addition, our results showed that HTLV‐1‐specific CD8+ T cells can be efficiently induced by OML/Tax from HTLV‐1 carriers compared with epitope peptide alone, and these HTLV‐1‐specific CD8+ T cells were able to lyse cells presenting the peptide. These results suggest that OML/Tax is capable of inducing antigen‐specific cellular immune responses without adjuvants and may be useful as an effective vaccine carrier for prophylaxis in tumors and infectious diseases by substituting the epitope peptide.