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Macromolecular NMR spectroscopy for the non‐spectroscopist
Author(s) -
Kwan Ann H.,
Mobli Mehdi,
Gooley Paul R.,
King Glenn F.,
Mackay Joel P.
Publication year - 2011
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2011.08004.x
Subject(s) - nuclear magnetic resonance spectroscopy , transverse relaxation optimized spectroscopy , spectroscopy , macromolecule , fluorine 19 nmr , two dimensional nuclear magnetic resonance spectroscopy , interpretation (philosophy) , chemistry , nuclear magnetic resonance , computer science , physics , biochemistry , quantum mechanics , programming language
NMR spectroscopy is a powerful tool for studying the structure, function and dynamics of biological macromolecules. However, non‐spectroscopists often find NMR theory daunting and data interpretation nontrivial. As the first of two back‐to‐back reviews on NMR spectroscopy aimed at non‐spectroscopists, the present review first provides an introduction to the basics of macromolecular NMR spectroscopy, including a discussion of typical sample requirements and what information can be obtained from simple NMR experiments. We then review the use of NMR spectroscopy for determining the 3D structures of macromolecules and examine how to judge the quality of NMR‐derived structures.