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CD91 interacts with mannan‐binding lectin (MBL) through the MBL‐associated serine protease‐binding site
Author(s) -
Duus Karen,
Thielens Nicole M.,
Lacroix Monique,
Tacnet Pascale,
Frachet Philippe,
Holmskov Uffe,
Houen Gunnar
Publication year - 2010
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2010.07901.x
Subject(s) - uniprot , collectin , ficolin , biochemistry , serine protease , chemistry , protease , lectin , masp1 , enzyme , mannan binding lectin , biology , innate immune system , receptor , gene
CD91 plays an important role in the scavenging of apoptotic material, possibly through binding to soluble pattern‐recognition molecules. In this study, we investigated the interaction of CD91 with mannan‐binding lectin (MBL), ficolins and lung surfactant proteins. Both MBL and L‐ficolin were found to bind CD91. The MBL–CD91 interaction was time‐ and concentration‐dependent and could be inhibited by known ligands of CD91. MBL‐associated serine protease 3 (MASP‐3) also inhibited binding between MBL and CD91, suggesting that the site of interaction is located at or near the MASP–MBL interaction site. This was confirmed by using MBL mutants deficient for MASP binding that were unable to interact with CD91. These findings demonstrate that MBL and L‐ficolin interact with CD91, strongly suggesting that they have the potential to function as soluble recognition molecules for scavenging microbial and apoptotic material by CD91. Structured digital abstract• MINT‐8040679 , MINT‐8040706 : MBL (uniprotkb: P11226 ) binds ( MI:0407 ) to CD91 (uniprotkb: Q07954 ) by enzyme linked immunosorbent assay ( MI:0411 ) • MINT‐8040690 : L‐ficolin (uniprotkb: Q15485 ) binds ( MI:0407 ) to CD91 (uniprotkb: Q07954 ) by enzyme linked immunosorbent assay ( MI:0411 ) • MINT‐8040663 : C1q B (uniprotkb: P02746 ), C1q C (uniprotkb: P02747 ), C1q A (uniprotkb: P02745 ) and CD91 (uniprotkb: Q07954 ) physically interact ( MI:0915 ) by enzyme linked immunosorbent assay ( MI:0411 ) • MINT‐8040821 , MINT‐8040869 , MINT‐8040928 : CD91 (uniprotkb: Q07954 ) binds ( MI:0407 ) to MBL (uniprotkb: P11226 ) by surface plasmon resonance ( MI:0107 ) • MINT‐8040880 : CD91 (uniprotkb: Q07954 ) binds ( MI:0407 ) to L‐ficolin (uniprotkb: Q15485 ) by surface plasmon resonance ( MI:0107 )