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Flexibility and communication within the structure of the Mycobacterium smegmatis methionyl‐tRNA synthetase
Author(s) -
Ingvarsson Henrik,
Unge Torsten
Publication year - 2010
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2010.07784.x
Subject(s) - mycobacterium smegmatis , allosteric regulation , transfer rna , binding site , chemistry , methionine , stereochemistry , biochemistry , adenosine , adenosine triphosphate , enzyme , amino acid , rna , gene , mycobacterium tuberculosis , medicine , tuberculosis , pathology
Two structures of monomeric methionyl‐tRNA synthetase, from Mycobacterium smegmatis , in complex with the ligands methionine/adenosine and methionine, were analyzed by X‐ray crystallography at 2.3 Å and at 2.8 Å, respectively. The structures demonstrated the flexibility of the multidomain enzyme. A new conformation of the structure was identified in which the connective peptide domain bound more closely to the catalytic domain than described previously. The KMSKS(301‐305) loop in our structures was in an open and inactive conformation that differed from previous structures by a rotation of the loop of about 90° around hinges located at Asn297 and Val310. The binding of adenosine to the methionyl‐tRNA synthetase methionine complex caused a shift in the KMSKS domain that brought it closer to the catalytic domain. The potential use of the adenosine‐binding site for inhibitor binding was evaluated and a potential binding site for a specific allosteric inhibitor was identified.