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Induction of Krüppel‐like factor 4 by high‐density lipoproteins promotes the expression of scavenger receptor class B type I
Author(s) -
Yang Tao,
Chen Caihong,
Zhang Bin,
Huang He,
Wu Ganqiu,
Wen Jianguo,
Liu Junwen
Publication year - 2010
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2010.07779.x
Subject(s) - scavenger receptor , klf4 , chromatin immunoprecipitation , microbiology and biotechnology , electrophoretic mobility shift assay , western blot , transcription factor , biology , chemistry , northern blot , transfection , promoter , gene expression , gene , biochemistry , cholesterol , lipoprotein , sox2
Krüppel‐like factor 4 (KLF4) is an evolutionarily conserved zinc finger‐containing transcription factor. In the present study, peripheral blood mononuclear cells and phorbol 12‐myristate 13‐acetate‐differentiated THP‐1 cells were treated with oxidized low‐density lipoproteins and high‐density lipoproteins to determine the expression of KLF4 and scavenger receptor class B type I (SR‐BI). A full‐length cDNA of KLF4 or short interference RNA against KLF4 was transfected into THP‐1 cells, and the subsequent expressions of SR‐BI were analysed by real‐time PCR and western blot. The binding and transcriptional activities of KLF4 to the SR‐BI promoter were detected by electrophoretic mobility shift assay, chromatin immunoprecipitation assay and luciferase reporter assay. The results showed that induction of KLF4 by high‐density lipoproteins could promote the expression of SR‐BI, resulting from the binding to putative KLF4 binding element on the promoter of SR‐BI. All results indicate a potential function of KLF4 in the pathogenesis of atherosclerosis through the regulation effect on atherosclerotic‐related genes.