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Neuronal growth‐inhibitory factor (metallothionein‐3): structure–function relationships
Author(s) -
Ding ZhiChun,
Ni FengYun,
Huang ZhongXian
Publication year - 2010
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2010.07716.x
Subject(s) - metallothionein , mutant , chemistry , inhibitory postsynaptic potential , molecular dynamics , function (biology) , biophysics , microbiology and biotechnology , computational biology , stereochemistry , biology , biochemistry , neuroscience , computational chemistry , gene
Neuronal growth‐inhibitory factor (GIF), also named metallothionein‐3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein‐engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif‐induced characteristic conformation, the solvent accessibility and dynamics of the metal–thiolate cluster, and the domain–domain interactions.