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Polypyrimidine tract‐binding protein is essential for early mouse development and embryonic stem cell proliferation
Author(s) -
Shibayama Masaki,
Ohno Satona,
Osaka Takashi,
Sakamoto Reiko,
Tokunaga Akinori,
Nakatake Yuhki,
Sato Mitsuharu,
Yoshida Nobuaki
Publication year - 2009
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2009.07380.x
Subject(s) - polypyrimidine tract binding protein , biology , embryonic stem cell , microbiology and biotechnology , messenger rna , translation (biology) , rna splicing , cell growth , stem cell , exon , cell cycle , polypyrimidine tract , cell , rna binding protein , rna , gene , genetics
Polypyrimidine tract‐binding protein (PTB) is a widely expressed RNA‐binding protein with multiple roles in RNA processing, including the splicing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site‐dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear. Here, we report that a homozygous mutation in the mouse Ptb gene causes embryonic lethality shortly after implantation. We also established Ptb −/− embryonic stem (ES) cell lines and found that these mutant cells exhibited severe defects in cell proliferation without aberrant differentiation in vitro or in vivo . Furthermore, cell cycle analysis and a cell synchronization assay revealed that Ptb −/− ES cells have a prolonged G 2 /M phase. Thus, our data indicate that PTB is essential for early mouse development and ES cell proliferation.