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Mammalian glutaminyl cyclases and their isoenzymes have identical enzymatic characteristics
Author(s) -
Stephan Anett,
Wermann Michael,
von Bohlen Alex,
Koch Birgit,
Cynis Holger,
Demuth HansUlrich,
Schilling Stephan
Publication year - 2009
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2009.07337.x
Subject(s) - isozyme , enzyme , biochemistry , chemistry
Glutaminyl cyclases (QCs) catalyze the formation of pyroglutamate residues at the N‐terminus of several peptides and proteins from plants and animals. Recently, isoenzymes of mammalian QCs have been identified. In order to gain further insight into the biochemical characteristics of isoQCs, the human and murine enzymes were expressed in the secretory pathway of Pichia pastoris . Replacement of the N‐terminal signal anchor by an α‐factor prepropeptide from Saccharomyces cerevisiae resulted in poor secretion of the protein. Insertion of an N‐terminal glycosylation site and shortening of the N‐terminus improved isoQC secretion 100‐fold. A comparison of different recombinant isoQC proteins did not reveal an influence of mutagenic changes on catalytic activity. An initial characterization showed identical modes of substrate conversion of human isoQC and murine isoQC. Both proteins displayed a broad substrate specificity and preference for hydrophobic substrates, similar to the related QC. Likewise, a determination of the zinc content and reactivation of the apo‐isoQC revealed equimolar zinc present in QC and isoQC. Far‐UV CD spectroscopic analysis of murine QC and isoQC indicated virtually identical structural components. The present investigation provides the first enzymatic characterization of mammalian isoQCs. QC and isoQC represent very similar proteins, which are both present in the secretory pathway of cells. The functions of QCs and isoQC probably complement each other, suggesting a pivotal role of pyroglutamate modification for protein and peptide maturation.