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The potassium channel subunit Kvβ3 interacts with pannexin 1 and attenuates its sensitivity to changes in redox potentials
Author(s) -
Bunse Stefanie,
Locovei Silviu,
Schmidt Matthias,
Qiu Feng,
Zoidl Georg,
Dahl Gerhard,
Dermietzel Rolf
Publication year - 2009
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2009.07334.x
Subject(s) - pannexin , potassium channel , immunoprecipitation , microbiology and biotechnology , protein subunit , ion channel , chemistry , biophysics , sodium channel , biology , gap junction , biochemistry , intracellular , receptor , sodium , organic chemistry , connexin , gene
Pannexin 1 (Panx1), a member of the second gap junction protein family identified in vertebrates, appears to preferentially form non‐junctional membrane channels. A candidate regulatory protein of Panx1 is the potassium channel subunit Kvβ3, previously identified by bacterial two‐hybrid strategies. Here, we report on the physical association of Panx1 with Kvβ3 by immunoprecipitation when co‐expressed in a neuroblastoma cell line (Neuro2A). Furthermore, in vivo co‐expression of Panx1 and Kvβ3 was shown to occur in murine hippocampus and cerebellum. Kvβ3 is known to accelerate inactivation of otherwise slowly inactivating potassium channels under reducing conditions. We subsequently found that Panx1 channel currents exhibit a significant reduction when exposed to reducing agents, and that this effect is attenuated in the presence of Kvβ3. Apparently, Kvβ3 is involved in regulating the susceptibility of Panx1 channels to redox potential. Furthermore, the Panx1 channel blockers carbenoxolone and Probenecid were less effective in inhibiting Panx1 currents when Kvβ3 was co‐expressed. The influence of Kvβ3 on Panx1 is the first example of modulation of Panx1 channel function(s) by interacting proteins, and suggests the physiological importance of sensing changes in redox potentials. Structured digital abstract• MINT‐7260843 , MINT‐7260856 : Panx1 (uniprotkb: Q9JIP4 ) physically interacts ( MI:0915 ) with Kvβ3 (uniprotkb: P97382 ) by anti‐tag co‐immunoprecipitation ( MI:0007 )