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A new and efficient method for inhibition of RNA viruses by DNA interference
Author(s) -
Nowak Monika,
Wyszko Eliza,
FedorukWyszomirska Agnieszka,
Pospieszny Henryk,
Barciszewska Mirosława Z.,
Barciszewski Jan
Publication year - 2009
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2009.07145.x
Subject(s) - small interfering rna , rna interference , rna , trans acting sirna , dicer , dna , rna silencing , dna directed rna interference , gene silencing , biology , microbiology and biotechnology , rna induced transcriptional silencing , virology , rna induced silencing complex , gene expression , gene , genetics
We report here a new method for inhibition of RNA viruses induced by dsDNA. We demonstrated that both long dsDNA molecules and short interfering DNA with a sequence complementary to that of viral RNA inhibited tobacco mosaic virus expression and prevented virus spread. Also, the expression of the HIV‐1 gp41 gene in HeLa cells was inhibited by complementary short interfering DNA. We showed that Dicer processed dsDNA, which suggests activation of the cellular machinery involved in silencing of RNA. For the silencing of viral RNA effected with dsDNA, we coined the term DNA interference technology.