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cAMP response element‐binding protein (CREB) is imported into mitochondria and promotes protein synthesis
Author(s) -
De Rasmo Domenico,
Signorile Anna,
Roca Emilio,
Papa Sergio
Publication year - 2009
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2009.07133.x
Subject(s) - creb , microbiology and biotechnology , cyclic amp response element binding protein , mitochondrion , chemistry , binding protein , biology , biochemistry , transcription factor , gene
The cAMP response element‐binding protein (CREB) is a ubiquitous transcription factor in the higher eukaryotes that, once phosphorylated, promotes transcription of cAMP response element‐regulated genes. We have studied the mitochondrial import of CREB and its effect on the expression of mtDNA‐encoded proteins. [ 35 S]Methionine‐labelled CREB, synthesized in vitro in the Rabbit Reticulocyte Lysate system using a construct of the human cDNA, was imported into the matrix of isolated rat liver mitochondria by a membrane potential and TOM complex‐dependent process. The imported CREB caused cAMP‐dependent promotion of the synthesis of mitochondrially encoded subunits of oxidative phosphorylation enzyme complexes. Thus, CREB moves from the cytosol to mitochondria, in addition to the nucleus, and, when phosphorylated by cAMP‐dependent protein kinase, promotes the expression of mitochondrial genes.