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Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae
Author(s) -
Stephan Octavian,
Koch Christian
Publication year - 2009
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2009.07095.x
Subject(s) - promoter , histone deacetylase , chromatin immunoprecipitation , saccharomyces cerevisiae , biology , microbiology and biotechnology , histone h4 , transcription factor , transcription (linguistics) , chromatin , histone , genetics , gene , gene expression , linguistics , philosophy
Saccharomyces cerevisiae cells control their cell size at a point in late G 1 called Start. Here, we describe a negative role for the Sin3/Rpd3 histone deacetylase complex in the regulation of cell size at Start. Initiation of G 1 /S‐specific transcription of CLN1 , CLN2 and PCL1 in a sin3Δ strain occurs at a reduced cell size compared with a wild‐type strain. In addition, inactivation of the transcriptional regulator SIN3 partially suppressed a cln3Δ mutant, causing sin3Δcln3Δ double mutants to start the cell cycle at wild‐type size. Chromatin immunoprecipitation results demonstrate that Sin3 and Rpd3 are recruited to promoters of SBF (Swi4/Swi6)‐regulated genes, and reveal that binding of Sin3 to SBF‐specific promoters is cell‐cycle regulated. We observe that transcriptional repression of SBF‐dependent genes in early G 1 coincides with the recruitment of Sin3 to specific promoters, whereas binding of Sin3 is abolished from Swi4/Swi6‐regulated promoters when transcription is activated at the G 1 to S phase transition. We conclude that the Sin3/Rpd3 histone deacetylase complex helps to prevent premature activation of the S phase in daughter cells.