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Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms
Author(s) -
Debrand Emmanuel,
El Jai Yasmine,
Spence Lorraine,
Bate Neil,
Praekelt Uta,
Pritchard Catrin A.,
Monkley Susan J.,
Critchley David R.
Publication year - 2009
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2009.06893.x
Subject(s) - gene isoform , exon , alternative splicing , biology , rna splicing , gene , integrin , microbiology and biotechnology , coding region , focal adhesion , signal transducing adaptor protein , genetics , cell , signal transduction , rna
Talins are large adaptor proteins that link the integrin family of adhesion molecules to F‐actin. In vertebrates, there are two talin genes. Talin 1 is essential for integrin‐mediated cell adhesion; the role of talin 2 is unclear. Here we report a detailed analysis of mammalian talin 2 . This reveals the existence of a previously unrecognized promoter associated with a CpG island, and separated from the first coding exon by numerous alternatively spliced noncoding exons spanning > 200 kb. Analysis of a mouse gene trap line shows that this promoter accounts for most of the talin 2 expression in adult tissues. We also demonstrate that testis and kidney express truncated talin 2 isoforms that lack the N‐terminal half of the protein, and provide evidence for the developmentally regulated expression of the short testis‐specific talin 2 isoform in elongating spermatids. Finally, we identify four tissue‐specific alternative splicing events within the coding region of talin 2 .