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Δ G ‐based prediction and experimental confirmation of SYCRP1‐binding sites on the Synechocystis genome
Author(s) -
Omagari Katsumi,
Yoshimura Hidehisa,
Suzuki Takayuki,
Takano Mitunori,
Ohmori Masayuki,
Sarai Akinori
Publication year - 2008
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2008.06618.x
Subject(s) - synechocystis , computational biology , genome , biology , genetics , gene , cyanobacteria , bacteria
DNA‐binding sites for SYCRP1, which is a regulatory protein of the cyanobacterium Synechocystis sp. PCC6803, were predicted for the whole genome sequence by estimating changes in the binding free energy () for SYCRP1 for those sites. The values were calculated by summing ΔΔ G values derived from systematic single base‐pair substitution experiments (symmetrical and cooperative binding model). Of the calculated binding sites, 23 sites with a value < 3.9 kcal·mol −1 located upstream or between the ORFs were selected as putative binding sites for SYCRP1. In order to confirm whether SYCRP1 actually binds to these binding sites or not, 11 sites with the lowest values were tested experimentally, and we confirmed that SYCRP1 binds to ten of the 11 sites with a ΔΔ G total value < 3.9 kcal·mol −1 . The best correlation coefficient between and the observed ΔΔG total for binding of SYCRP1 to those sites was 0.78. These results suggest that the ΔΔ G values derived from systematic single base‐pair experiments may be used to screen for potential binding sites of a regulatory protein in the genome sequence.