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Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae
Author(s) -
Jensen Mette M.,
Christensen Marianne S.,
Bonven Bjarne,
Jensen Torben H.
Publication year - 2008
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2008.06451.x
Subject(s) - chromatin , transcription (linguistics) , microbiology and biotechnology , heat shock factor , histone , transcription coregulator , biology , downregulation and upregulation , chemistry , gene , genetics , heat shock protein , chromatin remodeling , hsp70 , linguistics , philosophy
Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by the massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. In this article, we identify determinants of this reassembly throughout the heat shock protein 104 gene ( HSP104 ) transcription unit. The results show that, although histone H3 lacking amino acids 4–30 of its N‐terminal tail (H3Δ4–30) is normally deposited, reassembly of H3Δ4–40 is obliterated with an accompanying sustained transcription. On mutation of the histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redeposition and ongoing transcription is not required to prevent chromatin reassembly.