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Schizosaccharomyces pombe positive cofactor 4 stimulates basal transcription from TATA‐containing and TATA‐less promoters through Mediator and transcription factor IIA
Author(s) -
ContrerasLevicoy Juan,
Urbina Fabiola,
Maldonado Edio
Publication year - 2008
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2008.06429.x
Subject(s) - transcription factor ii a , general transcription factor , promoter , tata box , transcription factor ii b , tata binding protein , transcription factor ii d , transcription preinitiation complex , schizosaccharomyces pombe , tata box binding protein , rna polymerase ii , biology , transcription factor , transcription factor ii f , transcription factor ii h , microbiology and biotechnology , schizosaccharomyces , transcription factor ii e , genetics , gene , gene expression , saccharomyces cerevisiae
The positive cofactor 4 (PC4) protein has an important role in transcriptional activation, which has been proposed to be mediated by transcription factor IIA (TFIIA) and TATA‐binding protein‐associated factors. To test this hypothesis, we cloned the Schizosaccharomyces pombe PC4 gene and analysed the role of the PC4 protein in the stimulation of basal transcription driven by TATA‐containing and TATA‐less promoters. Sc. pombe PC4 was able to stimulate basal transcription from several TATA‐containing promoters and from the Initiator sequences of the highly transcribed Sc. pombe nmt1 gene. Moreover, it was demonstrated that Sc. pombe PC4 stimulates formation of the transcription preinitiation complex. Activation of transcription by PC4 was dependent on the Mediator complex and TFIIA, but was independent of TATA‐binding protein‐associated factor. PC4 binds to double‐stranded and single‐stranded DNA and interacts with TATA‐binding protein, TFIIB, TFIIA, Mediator, TFIIH and the transcriptional activator protein VP16.