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Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF‐1 – a molecular link between recombination and chromatin assembly during meiosis
Author(s) -
Ishii Satomi,
Koshiyama Akiyo,
Hamada Fumika N.,
Nara Takayuki Y.,
Iwabata Kazuki,
Sakaguchi Kengo,
Namekawa Satoshi H.
Publication year - 2008
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2008.06357.x
Subject(s) - homologous recombination , meiosis , biology , chromatin , genetic recombination , homologous chromosome , chromatin immunoprecipitation , synaptonemal complex , saccharomyces cerevisiae , recombinase , genetics , flp frt recombination , rad51 , microbiology and biotechnology , cohesin , recombination , dna , yeast , gene , promoter , gene expression
In eukaryotes, meiosis leads to genetically variable gametes through recombination between homologous chromosomes of maternal and paternal origin. Chromatin organization following meiotic recombination is critical to ensure the correct segregation of homologous chromosomes into gametes. However, the mechanism of chromatin organization after meiotic recombination is unknown. In this study we report that the meiosis‐specific recombinase Lim15/Dmc1 interacts with the homologue of the largest subunit of chromatin assembly factor 1 (CAF‐1) in the basidiomycete Coprinopsis cinerea ( Coprinus cinereus ). Using C. cinerea LIM15/DMC1 ( CcLIM15 ) as the bait in a yeast two‐hybrid screen, we have isolated the C. cinerea homologue of Cac1, the largest subunit of CAF‐1 in Saccharomyces cerevisiae , and named it C. cinerea Cac1‐like (CcCac1L). Two‐hybrid assays confirmed that CcCac1L binds CcLim15 in vivo . β‐Galactosidase assays revealed that the N‐terminus of CcCac1L preferentially interacts with CcLim15. Co‐immunoprecipitation experiments showed that these proteins also interact in the crude extract of meiotic cells. Furthermore, we demonstrate that, during meiosis, CcCac1L interacts with proliferating cell nuclear antigen (PCNA), a component of the DNA synthesis machinery recently reported as an interacting partner of Lim15/Dmc1. Taken together, these results suggest a novel role of the CAF‐1–PCNA complex in meiotic events. We propose that the CAF‐1–PCNA complex modulates chromatin assembly following meiotic recombination.