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Two novel Mesocestoides vogae fatty acid binding proteins – functional and evolutionary implications
Author(s) -
Alvite Gabriela,
Canclini Lucía,
Corvo Ileana,
Esteves Adriana
Publication year - 2008
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.06179.x
Subject(s) - biology , fatty acid binding protein , intron , gene , biochemistry , isoelectric point , genetics , cestoda , zoology , helminths , enzyme
This work describes two new fatty acid binding proteins (FABPs) identified in the parasite platyhelminth Mesocestoides vogae (syn. corti ). The corresponding polypeptide chains share 62% identical residues and overall 90% similarity according to clustalx default conditions. Compared with Cestoda FABPs, these proteins share the highest similarity score with the Taenia solium protein. M. vogae FABPs are also phylogenetically related to the FABP3/FABP4 mammalian FABP subfamilies. The native proteins were purified by chromatographical procedures, and apparent molecular mass and isoelectric point were determined. Immunolocalization studies determined the localization of the expression of these proteins in the larval form of the parasite. The genomic exon–intron organization of both genes is also reported, and supports new insights on intron evolution. Consensus motifs involved in splicing were identified.