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Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress
Author(s) -
Meistertzheim AnneLeila,
Tanguy Arnaud,
Moraga Dario,
Thébault MarieThérèse
Publication year - 2007
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.06156.x
Subject(s) - gill , crassostrea , heat shock protein , biology , oyster , suppression subtractive hybridization , gene , mantle (geology) , gene expression , shellfish , ostreidae , hsp70 , pacific oyster , microbiology and biotechnology , aquatic animal , ecology , genetics , fishery , fish <actinopterygii> , cdna library , paleontology
Groups of oysters ( Crassostrea gigas ) were exposed to 25 °C for 24 days (controls to 13 °C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 °C above the summer seawater temperature encountered in western Brittany, France where the animals were collected. Suppression subtractive hybridization was used to identify specific up‐ and downregulated genes in gill and mantle tissues after 7–10 and 24 days of exposure. The resulting libraries contain 858 different sequences that potentially represent highly expressed genes in thermally stressed oysters. Expression of 17 genes identified in these libraries was studied using real‐time PCR in gills and mantle at different time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle, showing that gills are more sensitive to thermal stress. Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3–7 days of exposure, followed by a decrease at 14 days, and a second, less‐pronounced increase at 17–24 days. A slow‐down in protein synthesis occurred after 24 days of thermal stress.