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Recruitment of coregulator complexes to the β‐globin gene locus by TFII‐I and upstream stimulatory factor
Author(s) -
CrusselleDavis Valerie J.,
Zhou Zhuo,
Anantharaman Archana,
Moghimi Babak,
Dodev Tihomir,
Huang Suming,
Bungert Jörg
Publication year - 2007
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.06128.x
Subject(s) - locus (genetics) , gene , upstream (networking) , globin , biology , genetics , microbiology and biotechnology , computer science , computer network
Upstream stimulatory factor and TFII‐I are ubiquitously expressed helix‐loop‐helix transcription factors that interact with E‐box sequences and or initiator elements. We previously demonstrated that upstream stimulatory factor is an activator of β‐globin gene expression whereas TFII‐I is a repressor. In the present study, we demonstrate that upstream stimulatory factor interacts with the coactivator p300 and that this interaction is restricted to erythroid cells expressing the adult β‐globin gene. Furthermore, we demonstrate that Suz12, a component of the polycomb repressor complex 2, is recruited to the β‐globin gene. Reducing expression of Suz12 significantly activates β‐globin gene expression in an erythroid cell line with an embryonic phenotype. Suz12 also interacts with the adult β‐globin gene during early stages of erythroid differentiation of mouse embryonic stem cells. Our data suggest that TFII‐I contributes to the recruitment of the polycomb repressor complex 2 complex to the β‐globin gene. Together, these data demonstrate that the antagonistic activities of upstream stimulatory factor and TFII‐I on β‐globin gene expression are mediated at least in part by protein complexes that render the promoter associated chromatin accessible or inaccessible for the transcription complex.