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Effect of oxidative stress and involvement of poly(ADP‐ribose) polymerase (PARP) in Dictyostelium discoideum development
Author(s) -
Rajawat Jyotika,
Vohra Iqbal,
Mir Hina A.,
Gohel Dhaval,
Begum Rasheedunnisa
Publication year - 2007
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.06083.x
Subject(s) - dictyostelium discoideum , poly adp ribose polymerase , oxidative stress , benzamide , microbiology and biotechnology , biology , oxidative phosphorylation , dna damage , reactive oxygen species , biochemistry , polymerase , chemistry , dna , stereochemistry , gene
Dictyostelium discoideum , a unicellular eukaryote, exhibits multicellularity upon nutrient starvation and is a good model system for developmental studies, and for the study of various signal transduction pathways. Reactive oxygen species at low doses act as signaling molecules; however, at high doses they are known to cause DNA damage that results in the activation of poly(ADP‐ribose) polymerase (PARP). We have earlier reported the high resistance of the unicellular stage of D. discoideum to oxidative stress, and we now show the response of this organism to oxidative stress and the role of PARP during development. We used hydroxylamine (HA) to induce in situ generation of H 2 O 2 and monitored the effect of benzamide, a PARP inhibitor, on oxidative stress‐induced changes in D. discoideum development. Interestingly, oxidative stress resulted in PARP activation within 5 min that was inhibited by benzamide. Oxidative stress‐induced delay in developmental pattern was also partially restored by benzamide. We studied the long‐term effects of PARP inhibition under oxidative stress, and our results demonstrated that spores formed under HA stress exhibited significant delay in germination in comparison to benzamide‐pretreated HA‐stressed cells. However, second‐generation cells showed normal development, signifying that PARP inhibition has no deleterious effect on D. discoideum development under oxidative stress.