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Identification and characterization of multiple species of tenascin‐X in human serum
Author(s) -
Egging D. F.,
Peeters A. C. T. M.,
Grebenchtchikov N.,
GeurtsMoespot A.,
Sweep C. G. J.,
den Heijer M.,
Schalkwijk J.
Publication year - 2007
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.05671.x
Subject(s) - identification (biology) , tenascin , characterization (materials science) , computational biology , biology , materials science , microbiology and biotechnology , nanotechnology , ecology , fibronectin , extracellular matrix
We analysed the diversity of tenascin‐X (TNX) species in serum and studied parameters that could affect determination of TNX levels in serum. Using western blot analysis we identified at least seven distinct TNX species, ranging from 75 kDa to the presumably full‐length 450 kDa form. Purification of serum TNX followed by sequence analysis positively identified two major TNX species of 75 and 140 kDa. We found that serum TNX binds to tropoelastin but not to fibrillar collagens. We conclude that serum TNX is composed of distinct molecular species that retain functional activity.