Premium
Direct identification of hydrophobins and their processing in Trichoderma using intact‐cell MALDI‐TOF MS
Author(s) -
Neuhof Torsten,
Dieckmann Ralf,
Druzhinina Irina S.,
Kubicek Christian P.,
NakariSetälä Tiina,
Penttilä Merja,
von Döhren Hans
Publication year - 2007
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.05636.x
Subject(s) - hypocrea , hydrophobin , trichoderma , trichoderma reesei , spore , biology , cell wall , mycelium , fungal protein , microbiology and biotechnology , mutant , botany , gene , biochemistry , enzyme , cellulase
Intact‐cell MS (ICMS) was applied for the direct detection of hydrophobins in various species and strains of Hypocrea / Trichoderma. In both mycelia and spores, dominating peaks were identified as hydrophobins by detecting mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain‐specific processing was observed in the case of Hypocrea jecorina (anamorph Trichoderma reesei ). An analysis of 32 strains comprising 29 different species of Trichoderma and Hypocrea showed hydrophobin patterns that were specific at both at the species and isolate (subspecies) levels. The method therefore permits rapid and direct detection of hydrophobin class II compositions and may also provide a means to identify Trichoderma (and other fungal) species and strains from microgram amounts of biomass without prior cultivation.