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Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti
Author(s) -
Lugo Juana M.,
Morera Yuliet,
Rodríguez Tania,
Huberman Alberto,
Ramos Laida,
Estrada Mario P.
Publication year - 2006
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2006.05555.x
Subject(s) - eyestalk , hemolymph , biology , complementary dna , litopenaeus , northern blot , shrimp , microbiology and biotechnology , molecular cloning , gene , endocrinology , genetics , biochemistry , hormone , fishery
The crustacean hyperglycemic hormone (CHH) plays an important role in the regulation of hemolymph glucose levels, but it is also involved in other functions such as growth, molting and reproduction. In the present study we describe the first CHH family gene isolated from the Atlantic Ocean shrimp Litopenaeus schmitti . Sequence analysis of the amplified cDNA fragment revealed a high nucleotide sequence identity with other CHHs. Northern blot analysis showed that the isolated CHH mRNA from L. schmitti is present in the eyestalk but not in muscle or stomach. We also investigated the ability of dsRNA to inhibit the CHH function in shrimps in vivo . Injection of CHH dsRNA into the abdominal hemolymh sinuses resulted in undetectable CHH mRNA levels within 24 h and a corresponding decrease in hemolymph glucose levels, suggesting that functional gene silencing had occurred. These findings are the first evidence that dsRNA technique is operative in adult shrimps in vivo .