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Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster
Author(s) -
Ruike Tatsushi,
Takeuchi Ryo,
Takata Keiichi,
Oshige Masahiko,
Kasai Nobuyuki,
Shimanouchi Kaori,
Kanai Yoshihiro,
Nakamura Ryoichi,
Sugawara Fumio,
Sakaguchi Kengo
Publication year - 2006
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2006.05504.x
Subject(s) - proliferating cell nuclear antigen , dna polymerase delta , dna clamp , biology , drosophila melanogaster , chromatin , microbiology and biotechnology , dna polymerase , dna replication , processivity , complementary dna , dna repair , dna , dna polymerase ii , gene , genetics , polymerase chain reaction , reverse transcriptase
The eukaryotic DNA polymerase processivity factor, proliferating cell nuclear antigen, is an essential component in the DNA replication and repair machinery. In Drosophila melanogaster , we cloned a second PCNA cDNA that differs from that encoded by the gene mus209 (for convenience called DmPCNA1 in this article). The second PCNA cDNA ( DmPCNA2 ) encoded a 255 amino acid protein with 51.7% identity to DmPCNA1 , and was ubiquitously expressed during Drosophila development. DmPCNA2 was localized in nuclei as a homotrimeric complex and associated with Drosophila DNA polymerase δ and ε in vivo . Treatment of cells with methyl methanesulfonate or hydrogen peroxide increased the amount of both DmPCNA2 and DmPCNA1 associating with chromatin, whereas exposure to UV light increased the level of association of only DmPCNA1. Our observations suggest that DmPCNA2 may function as an independent sliding clamp of DmPCNA1 when DNA repair occurs.