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Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics
Author(s) -
Boisvert Steve,
Joly David,
Carpentier Robert
Publication year - 2006
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2006.05475.x
Subject(s) - photosystem ii , fluorescence , chemistry , kinetic energy , kinetics , electron acceptor , electron transfer , acceptor , chlorophyll fluorescence , thylakoid , phase (matter) , analytical chemistry (journal) , biophysics , photochemistry , photosynthesis , physics , chromatography , chloroplast , biochemistry , biology , organic chemistry , optics , quantum mechanics , gene , condensed matter physics
Fluorescence induction has been studied for a long time, but there are still questions concerning what the O–J–I–P kinetic steps represent. Most studies agree that the O–J rise is related to photosystem II primary acceptor (Q A ) reduction, but several contradictory theories exist for the J–I and I–P rises. One problem with fluorescence induction analysis is that most work done to date has used only qualitative or semiquantitative data analysis by visually comparing traces to observe the effects of different chemicals or treatments. Although this method is useful to observe major changes, a quantitative method must be used to detect more subtle, yet important, differences in the fluorescence induction trace. To achieve this, we used a relatively simple mathematical approach to extract the amplitudes and half‐times of the three major fluorescence induction phases obtained from traces measured in thylakoid membranes kept at various temperatures. Apparent activation energies ( E A ) were also obtained for each kinetic step. Our results show that each phase has a different E A , with E A O–J