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Charging of tRNA with non‐natural amino acids at high pressure
Author(s) -
GielPietraszuk Malgorzata,
Barciszewski Jan
Publication year - 2006
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2006.05312.x
Subject(s) - aminoacylation , amino acid , transfer rna , chemistry , yield (engineering) , high pressure , ribose , stereochemistry , biochemistry , rna , enzyme , materials science , engineering physics , engineering , metallurgy , gene
We show a simple and reliable method of tRNA aminoacylation with natural, as well as non‐natural, amino acids at high pressure. Such specific and noncognate tRNAs can be used as valuable substrates for protein engineering. Aminoacylation yield at high pressure depends on the chemical nature of the amino acid used and it is up to 10%. Using CoA, which carries two potentially reactive groups ‐SH and ‐OH, as a model compound we showed that at high pressure amino acid is bound preferentially to the hydroxyl group of the terminal ribose ring.