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End‐damage‐specific proteins facilitate recruitment or stability of X‐ray cross‐complementing protein 1 at the sites of DNA single‐strand break repair
Author(s) -
Parsons Jason L.,
Dianova Irina I.,
Boswell Emma,
Weinfeld Michael,
Dianov Grigory L.
Publication year - 2005
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2005.04962.x
Subject(s) - ap site , dna ligase , dna damage , dna repair , dna , dna repair protein xrcc4 , dna ligases , biology , nucleotide excision repair , oligonucleotide , replication protein a , microbiology and biotechnology , endonuclease , base excision repair , biochemistry , dna binding protein , gene , transcription factor
Ionizing radiation, oxidative stress and endogenous DNA‐damage processing can result in a variety of single‐strand breaks with modified 5′ and/or 3′ ends. These are thought to be one of the most persistent forms of DNA damage and may threaten cell survival. This study addresses the mechanism involved in recognition and processing of DNA strand breaks containing modified 3′ ends. Using a DNA–protein cross‐linking assay, we followed the proteins involved in the repair of oligonucleotide duplexes containing strand breaks with a phosphate or phosphoglycolate group at the 3′ end. We found that, in human whole cell extracts, end‐damage‐specific proteins (apurinic/apyrimidinic endonuclease 1 and polynucleotide kinase in the case of 3′ ends containing phosphoglycolate and phosphate, respectively) which recognize and process 3′‐end‐modified DNA strand breaks are required for efficient recruitment of X‐ray cross‐complementing protein 1–DNA ligase IIIα heterodimer to the sites of DNA repair.

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