Premium
Structures of the O‐polysaccharides and classification of Proteus genomospecies 4, 5 and 6 into respective Proteus serogroups
Author(s) -
Zych Krystyna,
Perepelov Andrei V.,
Siwiňska Małgorzata,
Knirel Yuriy A.,
Sidorczyk Zygmunt
Publication year - 2005
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2005.04958.x
Subject(s) - proteus , proteus vulgaris , providencia , chemistry , polysaccharide , microbiology and biotechnology , proteus mirabilis , biology , escherichia coli , biochemistry , gene
An acidic branched O‐polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (LPS) of Proteus genomospecies 4 and studied by sugar and methylation analyses along with 1 H and 13 C NMR spectroscopy, including 2D COSY, TOCSY, ROESY and H‐detected 1 H, 13 C HSQC experiments. The following structure of the pentasaccharide repeating unit of the O‐polysaccharide was established, which is unique among Proteus polysaccharide structures: where Qui3NAc stands for 3‐acetamido‐3,6‐dideoxyglucose. Based on the O‐polysaccharide structure and serological data, we propose classifying Proteus genomospecies 4 into a new, separate Proteus serogroup, O56. A weak cross‐reactivity of Proteus genomospecies 4 antiserum with LPS of Providencia stuartii O18 and Proteus vulgaris OX2 was observed and is discussed in view of a similarity of the O‐polysaccharide structures. Structural and serological investigations showed that Proteus genomospecies 5 and 6 should be classified into the existing Proteus serogroups O8 and O69, respectively.