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Mitochondrial biogenesis in mtDNA‐depleted cells involves a Ca 2+ ‐dependent pathway and a reduced mitochondrial protein import
Author(s) -
Mercy Ludovic,
Pauw Aurélia de,
Payen Laetitia,
Tejerina Silvia,
Houbion Andrée,
Demazy Catherine,
Raes Martine,
Renard Patricia,
Arnould Thierry
Publication year - 2005
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2005.04913.x
Subject(s) - mitochondrial biogenesis , mitochondrial dna , mitochondrion , mitochondrial apoptosis induced channel , dnaja3 , biology , microbiology and biotechnology , tfam , atp–adp translocase , creb , mitochondrial fission , mitochondrial fusion , population , inner mitochondrial membrane , biochemistry , transcription factor , gene , demography , sociology
Alterations in mitochondrial activity resulting from defects in mitochondrial DNA (mtDNA) can modulate the biogenesis of mitochondria by mechanisms that are still poorly understood. In order to study mitochondrial biogenesis in cells with impaired mitochondrial activity, we used rho‐L929 and rho 0 143 B cells (partially and totally depleted of mtDNA, respectively), that maintain and even up‐regulate mitochondrial population, to characterize the activity of major transcriptional regulators (Sp1, YY1, MEF2, PPARgamma, NRF‐1, NRF‐2, CREB and PGC‐1α) known to control the expression of numerous nuclear genes encoding mitochondrial proteins. Among these regulators, cyclic AMP‐responsive element binding protein (CREB) activity was the only one to be increased in mtDNA‐depleted cells. CREB activation mediated by a calcium‐dependent pathway in these cells also regulates the expression of cytochrome c and the abundance of mitochondrial population as both are decreased in mtDNA‐depleted cells that over‐express CREB dominant negative mutants. Mitochondrial biogenesis in mtDNA‐depleted cells is also dependent on intracellular calcium as its chelation reduces mitochondrial mass. Despite a slight increase in mitochondrial mass in mtDNA‐depleted cells, the mitochondrial protein import activity was reduced as shown by a decrease in the import of radiolabeled matrix‐targeted recombinant proteins into isolated mitochondria and by the reduced mitochondrial localization of ectopically expressed HA‐apoaequorin targeted to the mitochondria. Decrease in ATP content, in mitochondrial membrane potential as well as reduction in mitochondrial Tim44 abundance could explain the lower mitochondrial protein import in mtDNA‐depleted cells. Taken together, these results suggest that mitochondrial biogenesis is stimulated in mtDNA‐depleted cells and involves a calcium‐CREB signalling pathway but is associated with a reduced mitochondrial import for matrix proteins.